Liquid Chromatography
High Performance Liquid Chromatography (HPLC), is one of the most widely used analytical techniques.Reasons for its widespread use are its sensitivity, its easy adaptability to quantitative determinations, its suitability for the separation of non-volatile or easily temperature-degradable compounds.
HPLC devices generally contain a pump, injector, column oven, detector and data logger system.Separation takes place in the column.The stationary phase consists of mm scaled particles, so high pressure pumps are needed to pass the mobile phase through the column.The chromatographic process begins with the injection of the sample into the column.The separation of components continues by pumping the analyte and mobile phase into the column.The detector response received for each separated component is displayed as a chromatogram on the computer screen.
There are 3 HPLC (Shimadzu)systems with 4 different detectors in our center. Available detectors are diode array detector (DAD), ultraviolet detector (UV), fluorescence detector (RF), and refractive index detector (RID).
| System 1 | System 2 | System 3 |
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Detector: DAD (SPD-M10Avp) Auto sampler: SIL–10AD vp System controller: SCL-10Avp Pump: LC-10ADvp Degasser: DGU-14A Column oven: CTO-10Avp |
Detector: RID 10A-DAD detector (SPD-M10Avp) Pump: LC-10ADvp Degasser: DGU-14A Column oven: CTO-10ACvp |
Detector: RF –10AXL Fluorescence Detector - UV detector(SPD-10AV vp) Auto sampler: SIL-20AC prominence System controller: LC-20AT prominence Pump: LC-20AT prominence Column oven: CTO-10AS vp |
Phenolic Compounds Analysis: Gallic Acid, Catechin, Catechol, Chlorogenic Acid, Caffeic Acid, Syringic Acid, Vanillin, Epicatechin, p-coumaric Acid, Ferulic Acid, o-coumaric Acid, Rutin, Resveratrol, Trans-cinnamic Acid, Quercetin, Vitexin, Naringin, Hesperidin, Apigenin-glucoside, Rosmarinic, Eriodictiol, Naringenin, Luteolin, Genistin, Apigenin, Carvacrol, Acecetin
Organic Acid Analysis: Ascorbic Acid, Fumaric Acid, Lactic Acid, Acetic Acid, Malic Acid, Succinic Acid, Tartaric Acid, Oxalic Acid, Formic Acid, Citric Acid, Pyruvic Acid
Sugar Analysis: Glucose, Fructose, Sucrose
Hormone Analysis: Abscisic acid, Indole-3-acetic acid
Biogenic Amine Analysis: Beta phenylethylamine, Tryptamine, Putrescine, Cadaverine, Tyramine
Tocopherol Analysis: Alpha-tocopherol, Beta-tocopherol, Gamma-tocopherol, Delta-tocopherol
Aflatoxin Analysis: B1, B2, G1, G2
Carotene Analysis: Alpha-carotene, Beta-carotene
HMF (Hydroxy Methyl Furfural) Analysis
Patulin Analysis
Lycopene Analysis
Amygdalin Analysis
Microcystin Analysis
Tannic acid Analysis
Astaxanthin Analysis
Benzoic acid Analysis
BPA(bisphenol A) Analysis
In addition to the existing analyses, requested analyzes can also be made.