Liquid Chromatography

‍High Performance Liquid Chromatography (HPLC),  is one of the most widely used analytical techniques.Reasons for its widespread use are its sensitivity, its easy adaptability to quantitative determinations, its suitability for the separation of non-volatile or easily temperature-degradable compounds.

HPLC devices generally contain a pump, injector, column oven, detector and data logger system.Separation takes place in the column.The stationary phase consists of mm scaled particles, so high pressure pumps are needed to pass the mobile phase through the column.The chromatographic process begins with the injection of the sample into the column.The separation of components continues by pumping the analyte and mobile phase into the column.The detector response received for each separated component is displayed as a chromatogram on the computer screen.

There are 3 HPLC (Shimadzu)systems with 4 different detectors in our center. Available detectors are diode array detector (DAD), ultraviolet detector (UV), fluorescence detector (RF), and refractive index detector (RID).

 

System 1 System 2 System 3

Detector: DAD (SPD-M10Avp)

Auto sampler: SIL–10AD vp

System controller: SCL-10Avp

Pump: LC-10ADvp

Degasser: DGU-14A

Column oven: CTO-10Avp

Detector: RID 10A-DAD detector (SPD-M10Avp)

Pump: LC-10ADvp

Degasser: DGU-14A

Column oven: CTO-10ACvp

Detector: RF –10AXL Fluorescence Detector - UV detector(SPD-10AV vp)

Auto sampler: SIL-20AC prominence

System controller: LC-20AT prominence

Pump: LC-20AT prominence

Column oven: CTO-10AS vp

 

Phenolic Compounds Analysis: Gallic Acid, Catechin, Catechol, Chlorogenic Acid, Caffeic Acid, Syringic Acid, Vanillin, Epicatechin, p-coumaric Acid, Ferulic Acid, o-coumaric Acid, Rutin, Resveratrol, Trans-cinnamic Acid, Quercetin, Vitexin, Naringin, Hesperidin, Apigenin-glucoside, Rosmarinic, Eriodictiol, Naringenin, Luteolin, Genistin, Apigenin, Carvacrol, Acecetin

Organic Acid Analysis: Ascorbic Acid, Fumaric Acid, Lactic Acid, Acetic Acid, Malic Acid, Succinic Acid, Tartaric Acid, Oxalic Acid, Formic Acid, Citric Acid, Pyruvic Acid

Sugar Analysis: Glucose, Fructose, Sucrose

Hormone Analysis: Abscisic acid, Indole-3-acetic acid

Biogenic Amine Analysis: Beta phenylethylamine, Tryptamine, Putrescine, Cadaverine, Tyramine

Tocopherol Analysis: Alpha-tocopherol, Beta-tocopherol, Gamma-tocopherol, Delta-tocopherol

Aflatoxin Analysis: B1, B2, G1, G2

Carotene Analysis: Alpha-carotene, Beta-carotene

HMF (Hydroxy Methyl Furfural) Analysis

Patulin Analysis

Lycopene Analysis

Amygdalin Analysis

Microcystin Analysis

Tannic acid Analysis

Astaxanthin Analysis

Benzoic acid Analysis

BPA(bisphenol A) Analysis

In addition to the existing analyses, requested analyzes can also be made.